CrestOptics V2tp & V2L-FOV Spinning Disk Imager

Description

The Crest Optics X-Light V2tp Confocal Imager is a full-spectrum spinning disk confocal imager that attaches to most major models of inverted or upright fluorescence microscope. It is designed to utilize the latest imaging technologies and is ideal for live cell imaging in biological applications.  The X-Light V2tp Confocal Imager allows imaging at the extremes of performance of a current‐generation scientific camera, and it provides for objectives used when imaging with a camera with a smaller detector.  It takes advantage of laser launches and LED light sources.  The result is a flexible confocal add‐on for existing microscopes that brings the latest commercialized technologies to microscopy.  The X-Light V2tp Confocal Imager is an extraordinary combination of capability and price.  A rapid motorized Disk In/DiskOut control provides access to widefield and confocal imaging modes.  Switching between modes is automated by imaging software.  A Nipkow Spinning Disk with proprietary disk pattern design provides high confocal resolution, improved out‐of‐focus rejection and higher ratio of signal‐to‐noise.  The result is < 800 nm confocal resolution with 60x 1.4 N.A. oil immersion objective.  Disks are available with a variety of pinholes sized for best results from different objectives: 40–μm holes, for objectives with N.A. < 1;  70–μm holes, for objectives with N.A. > 1.  Single Hole Pattern: 22×22–μm field of view for large chips of scientific CMOS cameras and Dual Hole Patterns: 12×12–μm field of view for each pattern, for flexibility for smaller chips of CCD cameras.  The disk rotates at 15,000 revolutions per minute, providing excitation and imaging at speeds of current cameras.  The excitation mount accommodates LED light sources or multimode fiber for lasers.  Its SMA connector provides high‐efficiency coupling and the gimbal mount for excitation eases alignment to produce the highest ratio of signal to noise while the motorized five‐position dichroic filter wheel and eight‐position emission filter wheel provide for automation and ease of interactive control.  The X-Light V2 L-FOV is the X-Light V2 variant which covers the latest very large field of view cameras introduced in the biological market.