CoolLED pE800

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  • On/off control, LED selection, Real time irradiance control
  • 4 channel wavelength-grouping design matches most commercially available dual, triple, and quad filters.
  • Removable inline excitation filter holders: no moving parts for fast acquisition
  • TTL and USB interfaces with major imaging software packages allows incorporation into all experimental parameters.
  • Internal function generator for electrophysiology and optogenetics applications.
  • Analogue inputs for dynamic irradiance control.
  • Instant on/off: No shutters required, no warm up or cool down with stable and repeatable: reliable and consistent results

Description

Featuring eight individually controllable LEDs and lightning fast TTL switching, the CoolLED pE-800 delivers the highest quality data with minimum total cost of ownership.  Versatile, intuitive and backed by CoolLED’s world-renowned support and a 36-month warranty, the pE-800 with liquid light guide delivery makes next generation LED illumination accessible to all.
Eight powerful and efficient LEDs offer broad spectral coverage from 365-740 nm for use with fluorophores ranging from DAPI to Cy7. The choice of fluorophore is no longer limited by the light source, and choosing the optimal set for each application has never been easier – even as requirements evolve over time.  All eight channels can be individually controlled, with each LED selected to perfectly match the selected fluorophores.  Excitation filters with white light sources let through more unwanted light, and by matching the LED this improves the signal to noise ratio for contrast-rich images revealing finer details and more information.  Industry-leading <7 µs TTL triggering is a breakthrough for live cell imaging.  Capturing dynamic events is possible with the highest temporal resolution and even without software, thanks to precise hardware synchronization via TTL input for each channel and compatibility with the pE-6501-8 (USB controlled TTL Conversion Kit).  The ability to fit inline excitation filters capitalizes on TTL trigger speeds, as single-band excitation filters can be installed for each channel. When used alongside multi-band filters, for example in a Pinkel configuration, this removes the need for expensive external filter wheels and overcomes the latency restrictions of mechanical movement, presenting a low-cost approach to high-speed imaging.  Affordable automation with Sequence Runner The popular Sequence Runner program takes speed one step further and combines the versatility of software with the <7 µs speeds of TTL, with minimal requirements.  Tight hardware synchronization not only increases temporal resolution, but also means samples are exposed only during acquisition, protecting against photobleaching and phototoxicity and pushing the boundaries of time-lapse studies.  In the Lightbridge graphical user interface, live samples can be further protected by balancing the irradiance to the lowest level possible while still maintaining image quality, with fine (0,1 %) control from 0-2 %, and in 1 % steps up to 100 %. And the more life-like a cell behaves, the more valuable a data set becomes.

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