CoolLED pE300

Description

The CoolLED’s pE-300white is a compact and simple to use LED illuminator for microscopes. It provides a broad spectrum of illumination, covering the excitation bands of common fluorophores such as DAPI, CFP, Aqua, FITC, TRITC, TxRed, Cy5 and many more. Operation is by a manual control pod with instant on/off and irradiance control from 0-100%, with remote control available via USB or TTL trigger.  The system is a mercury-free alternative that is safer, more controllable and precise than traditional bulb illumination options.  Spectral coverage is from the UV (DAPI excitation) to the red region (Cy5 excitation).  LED technology is quickly evolving, and since 2017 our new generation has double the irradiance at the sample plane, and for even higher irradiance is available as a Direct Fit model (which also reduces the cost to buy and run thanks to fewer consumables). With ACT Label certification, it’s even an environmentally friendly option for illumination.  There are two pE-300white configuration options: direct fit or liquid light guide. The direct fit variant delivers higher irradiance and reduces the cost to buy and run thanks to fewer consumables.  For confocal microscopes, the liquid light guide variant offers the ideal replacement to a metal halide light source, minimizing photobleaching and phototoxicity when viewing samples before scanning. More information on the available variants can be found under specifications.  An additional benefit of the pE-300white is that fluorophores can be viewed either individually or in combination, without filter cube changes. This makes it ideal for use with multi-band filter sets as the screening process can be simplified when fewer filter cubes are used. Independent control of the three LED wavebands allows control of the level of excitation of each fluorophore independently on a multi-stained sample, potentially removing the need for single-band filter sets altogether.

When it comes to making labs safer and more sustainable, LED microscope lighting is an attractive option when compared to old toxic mercury or metal halide lamps. All three channels of the pE-300white at full power use just 60 Watts, which is even lower than other LED technology which uses 120 to 350 Watts. “Green Grants” are even sometimes available from establishments, providing financial support owing to the reduction in institutional energy usage.